Bacteria are widespread in the environment, 医疗设备也不例外. 为了减少院内感染, 需要监测设备、仪器的污染情况, 还需要监测消毒灭菌设备的有效性. Correct sampling is to ensure that the monitoring results an important part. Throat swabs are used to wipe the patient’s throat during routine clinical testing. Throat swab sampling easy to use, with fewer errors, we will pharyngeal swab for monitoring equipment sampling.
Medico throat swab is a sterile product, radiation disinfection, shelf life of 2 年. The appearance of small plastic rods about 10 厘米, one is a nylon flocking fiber swab. Mainly used for clinical patients pharyngeal samples collected, but also for skin exudate, 伤口, epidermal specimens collected detection.
(1) bacterial swab test sampling method
Dry throat swab is used directly for rubbing, wet throat swab is used in the broth tube after rubbed. Sampling swab placed in the broth after full elution, the broth tube was placed in 37 Tuen training 24h. 37 Tuntang transfer plate, 24h culture. Take a single colony micro-enzyme, oxidase, coagulase experiments, while doing Gram stain, distinguish Gram-positive bacteria (G) and Gram-negative bacteria (G).
(2) Bacterial identification
Take a single colony of sterile cotton swabs to release 1.8 毫升 0. 45% NaCI sterile saline, make it into a bacterial suspension, the preparation of the concentration of No. 1 Macrobrachium (G bacteria) 或者 0. 5 Microbial cells (G bacteria) bacterial suspension inoculated with GN I card or GPI card. After inoculation of the card all released AN S system analysis.
Sampling can be used throat swabs or throat swabs can be used for bacterial contamination monitoring equipment sampling, 这种取样方法简单可靠, 但只为定性, 不能满足定量检测要求. 宴会期间, 其中许多都受到定性监测, indicating that aseptic or bacterial type can be determined.