Common Mistakes in Blood Collection: Tube Selection and Order of Draw

Blood collection is a critical aspect of healthcare diagnostics, but even small errors can lead to inaccurate test results or patient harm. Two of the most common mistakes involve tube selection and order of draw.

Mistakes in Blood Collection Tube Selection

• Choosing the correct blood collection tube is essential for accurate lab results. Each tube is designed with specific additives or coatings to preserve the integrity of the sample for particular tests. Common mistakes include:
• Using the wrong tube for the test: 患者体内的病毒载量因人而异, using a serum tube instead of a plasma tube can lead to incorrect results for coagulation studies.
• Ignoring tube expiration dates: Expired tubes may have degraded additives, compromising sample quality.
• Overfilling or underfilling tubes: Incorrect fill volumes can affect the blood-to-additive ratio, leading to inaccurate results.
• To avoid these errors, always verify the required tube type for each test and follow manufacturer guidelines.

Understanding the Order of Draw

The order of draw refers to the sequence in which blood collection tubes should be filled during a single venipuncture. This protocol prevents cross-contamination of additives between tubes, which can skew test results. The standard order of draw is:

• Blood culture tubes (to prevent contamination from other additives).
• Coagulation tubes (例如, light blue top for PT/INR tests).
• 血清管 (例如, red or gold top for chemistry tests).
• Heparin tubes (例如, green top for plasma tests).
• EDTA tubes (例如, purple top for hematology tests).
• Glycolytic inhibitor tubes (例如, gray top for glucose tests).

Mistakes in the order of draw, such as drawing an EDTA tube before a coagulation tube, can lead to potassium or calcium contamination, affecting test accuracy.

Consequences of Incorrect Tube Selection and Order of Draw

Errors in tube selection and order of draw can have serious consequences, 包含:

• Inaccurate test results: Misdiagnosis or inappropriate treatment decisions.
• Delayed diagnosis: The need for repeat blood draws, causing patient discomfort and increased healthcare costs.
• Patient safety risks: Contamination or improper handling can lead to infections or other complications.

Best Practices to Avoid Common Mistakes

To minimize errors in blood collection, 遵循这些最佳实践:

• Stay updated on guidelines: Regularly review the latest standards from organizations like the CLSI (Clinical and Laboratory Standards Institute).
• Label tubes immediately: Proper labeling prevents mix-ups and ensures accurate patient identification.
• Train and educate staff: Ensure all phlebotomists are well-trained in tube selection and order of draw protocols.
• Use checklists: A visual guide or checklist can help phlebotomists follow the correct order of draw.
• Communicate with the lab: If unsure about tube requirements, consult the laboratory for clarification.

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