咽拭子标本在手足口病监测中的应用

目的观察探讨咽拭子标本对手足口病的监测方法及效果 (手足口病) 并总结其临床应用价值. 方法 咽拭子标本 164 9月份以来我中心收集的手足口病患者 2009 到九月 2013 提取病毒RNA. 柯萨奇病毒型特异性引物 16 (考克斯A16) and enterovirus type 71(EV71) were detected by reverse transcription polymerase chain reaction (逆转录聚合酶链反应). Results Among 164 specimens, 90 案例 (54.9%) were positive for intestinal universal primers, 在这之中 83 案例 (50.6%) were positive for CoxA16 and 20 cases were positive for EV71. 12.2%. conclusion the epidemic monitoring of hand-foot-mouth disease showed that EV71 and CoxA16 viruses were the main pathogens, and throat swab specimens were used to monitor the epidemic situation of hand-foot-mouth disease, which was easy to operate, quick to produce results and high in detection rate, and had important clinical application value.

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