如何選擇合適的病毒傳輸介質

選擇合適的病毒傳播介質(虛擬機) 對於維持病毒 RNA 的完整性和確保準確的檢測結果至關重要. 常用的 VTM 有兩種類型 – 非滅活和滅活. 在本文中, 我們將討論如何選擇適合您需求的病毒傳輸介質.

MEIDIKE GENE® VTM Kit

Inactivated Virus Transport Medium

It is mainly a virus lysis type preservation solution improved by nucleic acid extraction lysis solution. The high concentration of guanidine salt in the sample can effectively inactivate the virus, which can effectively prevent the operator from secondary infection, but it also contains Rnase inhibitor, which can protect the viral nucleic acid from being degraded, so that the subsequent detection by NT-PCR can be performed. . 而且, it can be stored at room temperature for a relatively long time, which saves the cost of virus sample storage and transportation.

Non-inactivated Virus Transport Medium

It is mainly a virus maintenance liquid type preservation solution improved on the basis of the transport medium. It can preserve the activity of the virus in vitro and the integrity of antigens and nucleic acids, protect the viral protein coat from being easily decomposed, and maintain the originality of the virus sample to a large extent. In addition to nucleic acid extraction and detection, this preservation solution can also be used for virus culture, 隔離, and antigen detection, 等等。, but long-term storage after sampling requires strict low temperature.

Regardless of whether it is an inactivated type or a non-inactivated type, the virus sampling tube before sampling must be strictly inactivated and sterilized to ensure that there are no other microorganisms in the tube, which may cause the virus to decompose or other influences after sampling and cause false detection. 拭子取樣後, if a collection tool or preservation solution of poor quality is used, it will affect the subsequent test results and even cause misdiagnosis. 所以, you must choose a virus sampling tube produced by a professionally qualified manufacturer.