咽拭子標本在手足口病監測中的應用

Objective to observe and discuss the monitoring methods and effects of throat swab specimens on hand-foot-mouth disease (HFMD) and summarize its clinical application value. Methods The throat swab specimens of 164 patients with HFMD collected in our center from September 2009 to September 2013 were extracted with viral RNA. The specific primers of Coxsackie virus type 16 (CoxA16) and enterovirus type 71(EV71) were detected by reverse transcription polymerase chain reaction (逆轉錄聚合酶鏈反應). Results Among 164 specimens, 90 案例 (54.9%) were positive for intestinal universal primers, 在這之中 83 案例 (50.6%) were positive for CoxA16 and 20 cases were positive for EV71. 12.2%. conclusion the epidemic monitoring of hand-foot-mouth disease showed that EV71 and CoxA16 viruses were the main pathogens, and throat swab specimens were used to monitor the epidemic situation of hand-foot-mouth disease, which was easy to operate, quick to produce results and high in detection rate, and had important clinical application value.

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