ความรู้เบื้องต้นเกี่ยวกับหลักการตรวจหากรดนิวคลีอิกโดยผู้ผลิต Medico
We need scientific methods to diagnose or rule out whether a patient is infected with the new coronavirus.
ปัจจุบันมีวิธีการทดสอบวินิจฉัยที่เฉพาะเจาะจงและเชื่อถือได้, และมีเพียงหนึ่งเดียวเท่านั้น: การทดสอบกรดนิวคลีอิก.
1. Principles of conventional nucleic acid detection
Direction: วิธีการทางพันธุศาสตร์: RT-PCR Reverse transcription polymerase chain reaction Test object: Virus gene itself Accuracy: Maximum time: a few hours to a few days Advantages: High accuracy, is the only basis for diagnosis at present Disadvantages: Long time, High barriers to professional operation
The new coronavirus is an RNA virus that can replicate itself when it enters and infects the human body. As long as the person is infected, there must be a virus in the body. But because the virus is too small to be seen under the microscope, it cannot be confirmed directly by observation.
หลอดเก็บตัวอย่าง
Throat swab sample collection.
Everyone should be familiar with the term “ไม้กวาดคอ”, which is the primary source of samples for new coronavirus testing. Simply put, it is to use a plastic stick to reach the patient’s throat and wipe it, collect some respiratory fluid, then package it, refrigerate, and send it to the laboratory for RT-PCR testing.
อย่างไรก็ตาม, experts soon discovered that the results of the throat swab samples were inaccurate and the probability of false negatives was high. ดังนั้น, in different countries or regions, front-line medical workers may also collect samples such as nasal swabs, เสมหะ, and feces for testing.
throat swabsampling tube