Introduzione al principio del rilevamento dell'acido nucleico da parte dei produttori Medico

Introduzione al principio del rilevamento dell'acido nucleico da parte dei produttori Medico

We need scientific methods to diagnose or rule out whether a patient is infected with the new coronavirus.

Attualmente esistono metodi di test diagnostici specifici e autorevoli, e ce n'è solo uno: Test di acido nucleico.
1. Principles of conventional nucleic acid detection

Direction: Metodo genetico: RT-PCR Reverse transcription polymerase chain reaction Test object: Virus gene itself Accuracy: Maximum time: a few hours to a few days Advantages: High accuracy, is the only basis for diagnosis at present Disadvantages: Long time, High barriers to professional operation

The new coronavirus is an RNA virus that can replicate itself when it enters and infects the human body. As long as the person is infected, there must be a virus in the body. But because the virus is too small to be seen under the microscope, it cannot be confirmed directly by observation.

Throat swab sample collection.

Everyone should be familiar with the term “tampone faringeo”, which is the primary source of samples for new coronavirus testing. Simply put, it is to use a plastic stick to reach the patient’s throat and wipe it, collect some respiratory fluid, then package it, refrigerate, and send it to the laboratory for RT-PCR testing.

Tuttavia, experts soon discovered that the results of the throat swab samples were inaccurate and the probability of false negatives was high. Perciò, in different countries or regions, front-line medical workers may also collect samples such as nasal swabs, espettorato, and feces for testing.