आरटी-पीसीआर टेस्ट यानी रियल टाइम रिवर्स ट्रांसक्रिप्शन पोलीमरेज चेन रिएक्शन टेस्ट. RT-PCR परीक्षण प्रक्रिया का उपयोग SARS-CoV-2 से न्यूक्लिक एसिड का पता लगाने के लिए उन लोगों के नासॉफिरिन्जियल या ऑरोफरीन्जियल स्वैब नमूनों में किया जाता है, जिन्हें COVID-19 का निदान किया गया है।. यह प्रवर्धन प्रतिक्रिया की निगरानी के लिए प्रतिदीप्ति का उपयोग करके पूरा किया जाता है, एक तकनीक जिसे मात्रात्मक पीसीआर के रूप में जाना जाता है (qPCR). It allows professionals to immediately review the outcomes. One of the most extensively utilised methods for detecting COVID-19 is RT-PCR.
RT-PCR is a laboratory technique involving reverse transcription (आर टी) of viral RNA into DNA and then this Complementary DNA is amplified by the help of another technique called polymerase chain reaction (पीसीआर). It helps to detect even a very low concentration of virus at the time by amplification of DNA even when symptoms of the disease are not visible
What is the RT-PCR test procedure?
Sample collection is done using a swab to collect respiratory material found in your nose. he RT-PCR test identifies viral particles in patients using नासॉफिरिन्जियल स्वैब, ऑरोफरीन्जियल स्वैब, गले की सूजन, and nasal swabs.
With the use of an RT-PCR test kit, trained specialists perform the test. The swab is sealed in a tube and shipped to a covid-19 testing laboratory after collection.
It also involves the extraction of Covid-19 virus genetic material (शाही सेना). The PCR stage subsequently employs a PCR machine called a thermal cycler to amplify the genetic material of the SARS-CoV-2 virus. If the virus is present, one of the probes/chemicals creates a fluorescent light throughout this procedure
When should you get the test done
If you experience any one of the symptoms of corona virus, It is advisable to get the Covid-19 RT-PCR test done.
The most common Covid-19 symptoms are fever, dry cough, और थकान. सिर दर्द, ठंड लगना, disorientation, Muscle or joint discomfort, Loss of taste or smell, नाक बंद, Conjunctivitis (commonly known as red eyes), गला खराब होना, मतली या उलटी, different types of skin rash, or Diarrhea are some of the less common symptoms that may afflict some people.
Irritability, anxiety, depression, diminished consciousness, and sleeping difficulties are some of the mental symptoms that an infected person may experience.
People of all ages should seek medical attention right away if they have a fever and/or a cough that is accompanied by trouble breathing or shortness of breath, chest pain or pressure, or loss of speech or movement. If at all possible, contact your health care provider, hotline, or health facility first so that you can be directed to the appropriate clinic.
स्रोत: Internet
