Objective to observe and discuss the monitoring methods and effects of throat swab specimens on hand-foot-mouth disease (HFMD) and summarize its clinical application value. Methods The throat swab specimens of 164 patients with HFMD collected in our center from September 2009 to September 2013 were extracted with viral RNA. The specific primers of Coxsackie virus type 16 (CoxA16) and enterovirus …